The high-copy plasmids listed here contain mutated versions of this origin. * The pMB1 origin of replication is closely related to that of ColE1 and falls in the same incompatibility group. Origins of replication and copy numbers of various plasmids and cosmids A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture. A way to determine experimentally if the copy number of your plasmid is high or low is to perform a miniprep. This table can also be found online at the QIAGEN Plasmid Resource Center in the section ' Growth of bacterial cultures Plasmid Copy Number'. Transfection, cloning, sequencing, capillary sequencing, etc.įind out which origin of replication your plasmid contains, and look at the table below for classification into high-copy or low-copy types.
0 kommentar(er)
